Preparative ultracentrifugation - theory and practice

Aim

The performance of the preparative ultracentrifuge represents a most powerful technique. Properly employed, this technique may be used for isolation, separation and purification of a variety of particles from intact cells (e.g. viable mammalian cells and bacteria), cellular organelles (e.g. cytoplasmic vesicles, membrane fractions, mitochondria and nucleosomes), macromolecular nucleoprotein complexes (e.g. viruses and ribosomes), and unique macromolecules (e.g. proteins and nucleic acids). Carefully designed experiments may be carried out reproducibly only when having knowledge of the different parameters that affect the rate of sedimentation, the density properties of different particles in different centrifugation media, the unique properties of differently designed rotors, the relationship between the rotor speed and the relative centrifugal field, etc.

Location & Organization

Organizer

FLÄK - The Research School in Pharmaceutical Sciences

Course Director

Einar Everitt

Location / venue

Lund University (Faculty of Science)

Timing & Workload

Duration 1 week
ECTS points 3
Frequency Annual
 

Examination yes

Criteria

Is the course taught in English? yes
Is documentation available? (book, syllabus)? yes
Is the course open for external researchers? yes

More Information

http://www.biol.lu.se/cellorgbiol/postgrad/courses/course_24.html