Phage display and microarray technologies in biomedical research

Aim

In this one-week full-time course, you will learn about the practical and theoretical considerations of (i) phage display technology, (ii) DNA microarray technology, and (iii) antibody (protein) microarray technology. In addition, the course will provide insights into the principles of combinatorial biology.
Phage display is a powerful in vitro selection technology that enables us to specifically extract proteins with novel, desired properties from large protein libraries (more than 1010 members) generated by combinatorial biology. It provides a direct physical link between phenotype and genotype by utilizing the ability to display proteins on the surface of bacteriophages.
Molecular libraries play a central role within the novel DNA and protein microarray technologies. In these approaches, thousands of molecules (DNA or proteins) are dispended (pL scale) one by one in an ordered pattern, an array, onto a solid support (less than 1 cm2) and used as probes for the target analytes of interest. Numerous analytes can be analysed simultaneously in a single experiment. The array patterns generated can then be transformed into genomic or proteomic maps (fingerprints), revealing the detailed composition of the genome or proteome.
In the end, these methodologies will have a major impact on biomedicine, biotechnology, and high-throughput genomic and proteomic analysis for years to come, and will provide unique opportunities to perform e.g. disease diagnostics and biomarker discovery.

Location & Organization

Organizer

FLÄK - The Research School in Pharmaceutical Sciences

Course Director

Christer Wingren

Location / venue

Lund University (Faculty of Technology)

Timing & Workload

Duration 1 week
ECTS points 3
Frequency Annual
 

Examination yes

Criteria

Is the course taught in English? yes
Is documentation available? (book, syllabus)? yes
Is the course open for external researchers? yes

More Information

http://www.biol.lu.se/cellorgbiol/postgrad/courses/course_15.html