Imaging and 3D reconstruction with single particle cryo electron microscopy

Aim

The aim of the course is to give a theoretical and practical introduction to single particle imaging in cryo electron microscopy. This technique plays an important role in tying together structural information on an atomic level with models for structural organization and dynamics of organelles and cells. During the course it will be demonstrated how challenges in structural molecular biology can be addressed by combining electron microscopy and image processing in an interdisciplinary fashion. Course content:

Principles of the electron microscope and cryo electron microscopy.
Introduction to image processing.
Practical sessions in specimen preparation and electron microscopy in cryo or negative stain.
Image processing and 3D reconstruction in practice as computer based practicals.
Today's highlights and future trends.
By the interdisciplinary nature of the subject, some concepts of optics and applied mathematics will be introduced by necessity, while the intended audience is graduate students in biology, chemistry and life science with an interest in relations between structure and function on a subcellular level.

Location & Organization

Organizer

FLÄK - The Research School in Pharmaceutical Sciences

Course Director

Martin Lindahl

Location / venue

Lund University (Faculty of Science)

Timing & Workload

Duration 1 week
ECTS points 3
Frequency Annual
 

Examination yes

Criteria

Is the course taught in English? yes
Is documentation available? (book, syllabus)? yes
Is the course open for external researchers? yes

More Information

http://www.biol.lu.se/cellorgbiol/postgrad/courses/course_20.html