From genotype to phenotype: theory and practice in phenomics

Aim

This hands-on course addresses concepts, procedures and technical challenges when utilising mutant collections (e.g. gene-deletions) for high-throughput linking of genotypes to phenotypes on a genome-wide scale. The experimental part will focus around the yeast system for which such mutant collections are currently available, however, the potential for the use of genetic collections in other systems (bacterial, plants, animals) as well as the use of RNAi/morpholino for animals and cell cultures will be covered in lectures during the course.
The high-throughput phenotypic screening with yeast will include hands-on experience with robots for agar based analysis, liquid culture automated micro-cultivation as well as demonstration on mixed-population competition analysis with the use of the deletion-linked barcodes (utilising microarrays for analysis). The basics in genome-wide screens for synthetic lethality, Synthetic Genetic Arrays (SGA), will also be covered.
The course will in lectures and group work cover topics like large-scale deletion projects, standardisation, high-throughput phenotypic screening in micro-titre plates and some basic robot programming. Issues in high-throughput data analysis like precision, statistical significance and multiple testing will be covered. Central concepts in phenotyping like gene redundancy and gene-gene interactions (epistasis) will be discussed.

Location & Organization

Organizer

FLÄK - The Research School in Pharmaceutical Sciences

Course Director

Anders Blomberg

Location / venue

Göteborg University

Timing & Workload

Duration 1 week
ECTS points 3
Frequency Annual
 

Examination yes

Criteria

Is the course taught in English? yes
Is documentation available? (book, syllabus)? yes
Is the course open for external researchers? yes

More Information

http://www.biol.lu.se/cellorgbiol/postgrad/courses.html#