DNA amplification technology

Aim

DNA/RNA amplification and quantification in areas such as diagnostic PCR and molecular biology has been greatly improved by the introduction of real-time PCR technology. While this technology has tremendous potential for accurate and sensitive quantification, careful considerations regarding experimental design, sample preparation and reagent optimisation are required before it can be used correctly. The course will cover different aspects of polymerase chain reaction such as (i) absolute and relative quantification, (ii) conventional and real-time PCR, (iii) optimisation of PCR, (iv) primer/probe design, and (v) sample preparation and strategies to generate PCR-compatible samples.
The laboratory exercises will illustrate essential theoretical aspects of PCR and the results will be thoroughly discussed during the course. In the end of the course, we will mention various applications, such as gene expression, cloning, diagnostic PCR, mutagenesis, RAPD, RNA preparation etc, in the form of oral presentations and general discussions. The course spans five days and is held at the library and laboratories of Applied Microbiology (Kemicentrum). The number of participants is limited to eight.

Location & Organization

Organizer

FLÄK - The Research School in Pharmaceutical Sciences

Course Director

Peter Rådström

Location / venue

Lund University (Faculty of Technology)

Timing & Workload

Duration 1 week
ECTS points 3
Frequency Annual
 

Examination yes

Criteria

Is the course taught in English? yes
Is documentation available? (book, syllabus)? yes
Is the course open for external researchers? yes

More Information

http://www.biol.lu.se/cellorgbiol/postgrad/courses/course_16.html