Basic culturing of animal cells: theory and practice

Aim

Cell culturing is a very important tool for in vitro studies. This course includes both practical and theoretical knowledge of basic cell culturing techniques. The emphasis will be placed on a practical approach in laboratories equipped for research, mainly cell biological and molecular biology research. The course consists of lectures presenting the theory and practicals presenting a broad range of techniques. Among the techniques presented are routine culturing of cell lines, establishement of cell lines, freezing and thawing of cells, 96-well plate assays, detection of apoptosis, growth curves and growth kinetics, sampling of cells for various analyses and transfection. The students will be required to give a short presentation of their present or future field of research and how cell culture methodology is or will be used. The course leaders will also give a short presentation of their research and how cell culture methodology is used. A completed course gives the students sufficient knowledge to understand the principles of cell culturing and to be able to use this experimental system optimally on both a theoretical and pratical basis.

Location & Organization

Organizer

FLÄK - The Research School in Pharmaceutical Sciences

Course Director

Stina Oredsson

Location / venue

Lund University (Faculty of Science)

Timing & Workload

Duration 1 week
ECTS points 3
Frequency Annual
 

Examination yes

Criteria

Is the course taught in English? yes
Is documentation available? (book, syllabus)? yes
Is the course open for external researchers? yes

More Information

http://www.biol.lu.se/cellorgbiol/postgrad/courses/course_13.html